Microdetermination of glutamic acid

Simple microdetermination of uric acid.

In 1947 Kalckar introduced a comprehensive differential spectrophotometric system for the analysis of purines, including uric acid (1). Praetorius and his associate Poulsen employed this procedure and have published detailed directions for a practical uric acid method (2, 3). During the past 4 years, over 2000 routine uric acid determinations on blood and urine have been performed in this labor...

Determination of Glutamic Acid

Glutamic Acid Dehydrogenase and Glutamic-oxalacetic

As part of a larger project dealing with an in vestigation of amino acid and protein metabolism in leukemia, a study was undertaken to determine those enzymes concerned with amino acid anabo lism and catabolism as they occur in selected tis sues. Several review papers (@,4, 11, 1@)have ade quately summarized our present knowledge of the metabolism of white blood cells (WBC) in leuke mia. Howeve...

Microdetermination of alpha-keto acids with special reference to malic, lactic, and glutamic dehydrogenases in brain.

Previous studies have indicated marked differences in the quantitative distribution of a number of enzymes, as well as other substances, in various histologically distinct areas of the nervous system (l-4). An earlier method (2) for MDH, LDH, and GDH’ in brain required 0.3, 1.5, and 10 y of brain, dry weight, respectively, for the determination of these enzymatic activities. Some of the histolo...

Differential Presentation of Glutamic Acid